Monitoring E. coli Cultures with the BioLector and Multisizer 4e Instruments

Escherichia coli (E. coli) is a facultative anaerobic bacterium that lives in the lower intestine of warm-blooded animals, including humans.1 E. coli can be cultured easily and inexpensively in a laboratory setting and has become an important model organism in genetics, microbiology and biotechnology.1,2 E. coli is the most common organism used for the large-scale production of therapeutic proteins. Indeed, 30% of approved therapeutic proteins are currently being produced using E. coli.2 This application note will demonstrate how the BioLector microbioreactor and the Multisizer 4e Coulter Counter can be used to optimize E. coli culture conditions and characterize cell growth

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