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Mercodia Glucagon ELISA – 10 μL is a highly specific and sensitive immunoassay for measuring glucagon in serum, EDTA plasma, and cell culture media samples from rat, mouse, and non-human primates.
Kit format: 1 x 96 wells
Measurement range: 2-180 pmol/L (7 – 627 pg/mL)
Low sample volume – 10 µL
No purification of samples
Sample types: Serum, EDTA Plasma, and cell-culture medium
Measurement range: 2 – 180 pmol/L (7 – 627 pg/mL)
Detection limit: 1.5 pmol/L (5.23 pg/mL)
Little or no cross-reactivity to oxyntomodulin, glicentin, mini-glucagon, GLP-1, GLP-2 and GRPP
Highly appreciated and often cited assay
Test Characteristics
Samples: Serum, EDTA plasma and cell culture medium.
Sample volume: 10 µL
Assay range: 2-180 pmol/L (7 – 627 pg/mL)
Detection limit: 1.5 pmol/L
Incubation: 18-22 h (overnight) + 15 min
Detection method: absorbance
Specificity
Mini-glucagon, n.d.
Glicentin, human 1.0 %
Glicentin, mouse 7.0 %
Glicentin, rat 4.0 %
Oxyntomodulin, human/rat/mouse 2.0 %
Oxyntomodulin, bovine/canine/porcine n.d.
GLP-1 (7-36), n.d.
GLP-1 (9-36), n.d.
GLP-2, n.d.
For more information on Glucagon 10ul ELISA (10-1281-01) talk to Diagenics Ltd
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