Oxidised LDL ELISA (10-1143-01)

The Mercodia Oxidized LDL ELISA kit is intended to be used for the in vitro quantitative measurement of oxidized low density lipoproteins (oxidized LDL) in human serum or plasma. Kit format: 1 x 96 wells Measurement range: 1.4 – 21.3 mU/L (9 – 140 U/L when multiplied with dilution factor) Gives an accurate measurement of the physiological signalling to the scavenging macrophages that will create foam cells, vascular inflammation and atherosclerosis. CE/IVD labeled on select markets Controls included in the kit Test Characteristics Samples: Serum, plasma Sample volume: 25 µL Assay range: 1.4-21.3 mU/L Detection limit: ≤ 1 mU/L Incubation: 2 h +1 h + 15 min Detection method: absorbance Specificity The proprietary mouse monoclonal antibody 4E6 is developed by professors Holvoet and Collen at the University of Leuven in Belgium. It is is directed against a conformational epitope in the ApoB100 moiety of LDL that is generated as a consequence of substitution of at least 60 lysine residues of Apo B100 with aldehydes (Holvoet 2004). This number of substituted lysines corresponds to the minimal number required for scavenger-mediated uptake of oxidized LDL. Substituting aldehydes can be produced by peroxidation of lipids of LDL, resulting in the generation of oxidized LDL. However, lipid peroxidation is not required. Indeed, aldehydes that are released by endothelial cells under oxidative stress or by activated platelets may also induce the oxidative modification of Apo B100 in the absence of lipid peroxidation of LDL. The unique affinity of the antibody 4E6 used in the Mercodia Oxidized LDL ELISA makes it possible to measure both MDA and aldehyde modified oxidized LDL, which sets this assay apart from assays based on other antibodies.

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