Mouse Insulin ELISA (10-1247-01/10)

The Mercodia Mouse Insulin ELISA is an easy-to-use assay based on highly specific monoclonal antibodies with insignificant or no cross-reactivity to C-peptide or proinsulin. Kit format: 1 x 96 wells or 10 x 96 wells Measurement range: 0.2 – 6.5 µg/L Low sample volume – 10µL (5µL assay protocol available) Easy assay protocol (Shortened – 35 min – assay protocol available) Insignificant or no cross-reactivity to mouse C-peptide or proinsulin Test Characteristics Samples: Serum, EDTA plasma and cell culture medium. Sample volume: 10 µL (5 µL protocol available) Assay range: 0.2 – 6.5 µg/L Detection limit: ≤ 0.2 µg/L Incubation: 120 + 15 min Specificity The assay measures mouse insulin at 100 % . Mouse C-peptide I n.d. Mouse C-peptide II n.d. Rat C-peptide I n.d. Rat C-peptide II n.d. Rat insulin 146 % Mouse proinsulin I 43 % Mouse proinsulin II 60 % Rat proinsulin I 14 % Rat proinsulin II 60 % Human insulin 195 % Human proinsulin 82 % Human C-peptide n.d

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