Porcine C-peptide ELISA (10-1256-01)

An assay for the quantitative determination of porcine C-peptide in serum, plasma and cell culture medium. Kit format: 1 x 96 wells Measurement range: 20-1200 pmol/L No cross reactivity to human proinsulin or insulin No cross reactivity to C-peptide in human, macaque, rat or mouse High specificity – enables specific measurement of porcine C-peptide in xenotransplantation studies Test Characteristics Samples: Serum, plasma and cell culture medium Sample volume: 10 µL Assay range: 20-1200 pmol/L Detection limit: 10 pmol/L Incubation: 2 h + 1 h + 15 min Detection method: absorbance Specificity The following cross-reactions have been tested: Porcine insulin < 0.05 % Porcine proinsulin < 0.02 % Human C-peptide < 0.003 % Human insulin < 0.002 % Human proinsulin < 0.01 % Macaque C-peptide < 0.006 % Mouse insulin < 0.13 % Mouse proinsulin < 0.002 % Rat C-peptide 0.08 % Rat insulin < 0.002 % Rat proinsulin < 0.002 %

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