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Mercodia Total GLP-1 NL-ELISA provides a chemiluminescent method for the quantitative determination of amidated GLP-1 (glucagon-like-peptide-1) in human serum or plasma samples.
Kit format: 1×96 wells
Measurement range: 0.9 – 940 pmol/L (2.8 – 2940 pg/mL)
100% specificity to the predominating isoform, GLP-1 (9-36) amide
High sensitivity
Validated according to CLSI/FDA/EMA
Test Characteristics
Samples: Human serum and EDTA plasma
Sample volume: 25 µL
Assay range: 1 – 940 pmol/L
Detection limit: 0.65 pmol/L
Incubation: 2h + 15 min
Detection method: chemiluminescence
Specificity
The following cross-reactions have been tested:
GLP-1 (1-36) amide 88%
GLP-1 (7-36) amide 93%
GLP-1 (9-36) amide 100%
GLP-1 (1-37) n.d.
GLP-1 (7-37) n.d.
Glucagon n.d.
GIP n.d.
GLP-2 n.d.
Oxyntomodulin n.d.
Glicentin n.d.
Miniglucagon n.d.
MPGF n.d.
Liraglutide n.d.
Exenatide n.d.
Lixisenatide n.d.
Dulaglutide 0.07%
n.d. = not detectable
For more information on Total GLP-1 NL-ELISA (10-1278-01) talk to Diagenics Ltd
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