Closed Cell Respirometry

The closed cell method is generally more sensitive than the flow-through method to changes in respiration rate and has a faster response time. However, the main drawback of the closed-cell method is that as the experiment proceeds the organism will deplete the oxygen level inside the cell. If the oxygen concentration within the cell is allowed to drop below a certain threshold level then the organism’s behaviour and hence respiration rate may be affected. However, running shorter experiments and stopping to flush the cell out, before the critically low oxygen level is reached, then refilling with oxygen-saturated water could combat this.

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