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Flow-through respirometry is slightly more complicated than closed-cell, as the researcher will have to establish the correct balance in the flow rate to detect and measure changes in respiration without forming a significant concentration gradient within the chamber.
However the flow-through method ensures that the oxygen concentration remains constant throughout the course of the experiment and negates the requirement to refill the chamber with fresh water. Therefore, once the researcher has established the correct flow-rate, the experiment can essentially proceed over a much greater timescale without the concern of the oxygen concentration, or indeed any waste products, affecting the organism's behaviour.
For more information on Flow-Through Respirometry talk to Strathkelvin Instruments Limited
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